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91.
92.
Euglena gracilis makes two small Cd-binding proteins/peptides (Cd-BP I and Cd-BP II) in response to exposure to Cd2+. These proteins migrate slower than mammalian Cd-metallothionein (Cd-Mt) through Sephadex G-75, but like Cd-Mt also contain Zn and are separated into two species when chromatographed over a DEAE column. Both BPs are much less stable than Cd-Mt under acidic conditions. One source of this instability is that at least Cd-BP II contains acid-labile sulfide co-ordinated to the metals. It has been quantitated in Cd-BP II and shown to have a value of 1·25 ± 0·10 S2−/Cd. The reactivity of the Cd-binding sites in ligand substitution and thioldisulfide exchange reactions has been assessed. The reactions of Cd-BP I and II with pyridylazoresorcinol (PAR) were slow and displayed complex kinetic behavior. Similarly, both Cd-BPs react slowly and with complicated kinetics with 5,5′-dithiobis-(2-nitrobenzoate).  相似文献   
93.
Zooplankton diel vertical migration is evident on the mixed isothermal side of the western Irish Sea frontal system but is often influenced by large tides and persistent geostrophic currents. On the stratified side of the front, temperature acts as a controlling factor with most of the zooplankton occurring above the thermocline and carrying out pronounced vertical migration when chlorophyll a levels are low and diffuse. At higher chlorophyll levels, when discrete chlorophyll a maxima form, zooplankton vertical movement may be greatly modified with a large number of species and stages concentrating within these maxima at all times of the diel light cycle.  相似文献   
94.
Variation in glycogen concentration, condition index (CI) and filtration activity were measured in the bivalve Macoma balthica buried in sediment and experimentally exposed to cadmium (Cd). The stress due to elevated but sub-lethal concentrations (300 ppb Cd) affected the overall fitness of the organism as all parameters monitored responded significantly. Lower concentrations tested (10, 30 and 100 ppb) only induced a significant decrease in filtration activity, which may play a protective role, enabling the organism to slow down its metabolic activity and preserving the integrity of its reserves (reflected by stable CI and glycogen levels). Hence, the various endpoints selected show different thresholds. Our results also demonstrate that under high exposure, small individuals loose proportionally more glycogen per unit of weight than larger ones, thus confirming the higher sensitivity of small individuals to metal contamination. Furthermore, exposure to intermediate concentration (30 ppb) seems to be beneficial to the small individuals as indicated by their high CI values compared to the control. These results showed thus that non-sigmoidal concentration-response relationship and sizes of individuals should be considered in monitoring programmes and risk assessment.  相似文献   
95.
96.
The sources and distribution of organic matter (OM) in surface waters and sediments from Winyah Bay (South Carolina, USA) were investigated using a variety of analytical techniques, including elemental, stable isotope and organic biomarker analyses. Several locations along the estuary salinity gradient were sampled during four different periods of contrasting river discharge and tidal range. The dissolved organic carbon (DOC) concentrations of surface waters ranged from 7 mg l−1 in the lower bay stations closest to the ocean to 20 mg l−1 in the river and upper bay samples. There was a general linear relationship between DOC concentrations and salinity in three of the four sampling periods. In contrast, particulate organic carbon (POC) concentrations were significantly lower (0.1–3 mg l−1) and showed no relationship with salinity. The high molecular weight dissolved OM (HMW DOM) isolated from selected water samples collected along the bay displayed atomic carbon:nitrogen ratios ([C/N]a) and stable carbon isotopic compositions of organic carbon (δ13COC) that ranged from 10 to 30 and from −28 to −25‰, respectively. Combined, such compositions indicate that in most HMW DOM samples, the majority of the OM originates from terrigenous sources, with smaller contributions from riverine and estuarine phytoplankton. In contrast, the [C/N]a ratios of particulate OM (POM) samples varied significantly among the collection periods, ranging from low values of 5 to high values of >20. Overall, the trends in [C/N]a ratios indicated that algal sources of POM were most important during the early and late summer, whereas terrigenous sources dominated in the winter and early spring.In Winyah Bay bottom sediments, the concentrations of the mineral-associated OM were positively correlated with sediment surface area. The [C/N]a ratios and δ13COC compositions of the bulk sedimentary OM ranged from 5 to 45 and from −28 to −23‰, respectively. These compositions were consistent with predominant contributions of terrigenous sources and lesser (but significant) inputs of freshwater, estuarine and marine phytoplankton. The highest terrigenous contents were found in sediments from the river and upper bay sites, with smaller contributions to the lower parts of the estuary. The yields of lignin-derived CuO oxidation products from Winyah Bay sediments indicated that the terrigenous OM in these samples was composed of variable mixtures of relatively fresh vascular plant detritus and moderately altered soil OM. Based on the lignin phenol compositions, most of this material appeared to be derived from angiosperm and gymnosperm vascular plant sources similar to those found in the upland coastal forests in this region. A few samples displayed lignin compositions that suggested a more significant contribution from marsh C3 grasses. However, there was no evidence of inputs of Spartina alterniflora (a C4 grass) remains from the salt marshes that surround the lower sections of Winyah Bay.  相似文献   
97.
地学研究对海岸带综合治理的部分贡献   总被引:1,自引:1,他引:1  
地学是地质学、地球物理学和自然地理学的专称。运用地学手段对海岸带进行综合治理(ICZM)是当前海岸带持续开发战略面临的一项重要任务。“可持续”发展意思就是在保护环境的前提下对海岸地区及其资源进行管理,并要把它当成一个长期的具体任务来完成。要想进行ICZM必须要有广博的知识,需要了解海洋和海岸岩石的成因和特征、沉积物和地形的特征,还要了解天气和气候、海洋水体以及海洋中包含的生物等。海岸体系这些组成部分的基本地学知识是ICZM得以实施的基础,因为地学可以帮助明确再生和非再生资源以及它们对经济发展的潜在作用,从人类健康、安全和生物环境保护这两个方面弄清和确定经济发展已经和将要对环境产生的影响,特别从人类健康和工程风险这些方面弄清和确定发展过程中会有哪些限制因素和灾害;通过环境基本条件和监控变化来区分自然环境变化和人类活动导致的变化等。  相似文献   
98.
99.
Mercury speciation and its distribution in surface and deep waters of the Mediterranean Sea were studied during two oceanographic cruises on board the Italian research vessel URANIA in summer 2003 and spring 2004 as part of the Med Oceaneor and MERCYMS projects. The study included deep water profiles of dissolved gaseous Hg (DGM), reactive Hg (RHg), total Hg (THg), monomethyl Hg (MeHg) and dimethyl Hg (DMeHg) in open ocean waters. Average concentrations of measured Hg species were characterized by seasonal and spatial variations. Overall average THg concentrations ranged between 0.41 and 2.65 pM (1.32 ± 0.48 pM) and were comparable to those obtained in previous studies of the Mediterranean Sea. A significant fraction of Hg was present as “reactive” Hg (average 0.33 ± 0.32 pM). Dissolved gaseous Hg (DGM), which consists mainly of Hg0, represents a considerable proportion of THg (average 20%, 0.23 ± 0.11 pM). The portion of DGM typically increased towards the bottom, especially in areas with strong tectonic activity (Alboran Sea, Strait of Sicily, Tyrrhenian Sea), indicating its geotectonic origin. No dimethyl Hg was found in surface waters down to the depth of 40 m. Below this depth, its average concentration was 2.67 ± 2.9 fM. Dissolved fractions of total Hg and MeHg were measured in filtered water samples and were 0.68 ± 0.43 pM and 0.29 ± 0.17 pM for THg and MeHg respectively. The fraction of Hg as MeHg was in average 43%, which is relatively high compared to other ocean environments. The concentrations reported in this study are among the lowest found in marine environments and the quality of analytical methods are of key importance. Speciation of Hg in sea water is of crucial importance as THg concentrations alone do not give adequate data for understanding Hg sources and cycling in marine environments. For example, photoinduced transformations are important for the presence of reactive and elemental mercury in the surface layers, biologically mediated reactions are important for the production/degradation of MeHg and DGM in the photic zones of the water column, and the data for DGM in deep sea indicate the natural sources of Hg in geotectonicaly active areas of the Mediterranean Sea.  相似文献   
100.
We studied interactions of nitromusk compounds musk ketone and musk xylene and polycyclic musks Galaxolide trade mark (HHCB), Celestolide trade mark (ADBI), Tetralide trade mark (AHTN), and Traseolide trade mark (AITI) with multixenobiotic resistance (mxr) transporters in gill tissue of the marine mussel Mytilus californianus (Conrad, 1837). A competitive substrate transport test with rhodamine B was used to assay modulation of transport activity by musks. All tested musks inhibited the transport activity in the low microm range as indicated by increased accumulation of rhodamine B in the tissue. Compared to known substrates of mxr transporters, the effective concentration range was similar to quinidine and about 100 times higher than verapamil. Musk ketone and musk xylene also inhibited efflux of rhodamine B from gill tissue which was loaded with the dye and subsequently incubated with these compounds. Synthetic musk compounds are persistent environmental pollutants in aquatic environments with a high potential to bioaccumulate. As potent inhibitors of mxr transporters they may also play a role as chemosensitizers that enable toxic mxr substrates to accumulate in cells of aquatic organisms.  相似文献   
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